Streptomycin ELISA Kit

Streptomycin ELISA Kit is a competitive ELISA kit to detect Streptomycin and dihydrostreptomycin in milk, milk powder, honey and cell culture samples.

Streptomycin is an aminoglycoside antibiotic used in apiculture to protect bees against a variety of brood diseases. In veterinary practice, it is used to treat bacterial diseases in cattle, pigs, sheep, and poultry. Brazilian authorities have included it in the National Regulatory Monitoring Program for honey production. 

This current Streptomycin ELISA Kit is based on indirect competitive ELISA to detect Streptomycin residue in cell culture, milk, and honey. In the kit, streptomycin antigen is coated on the microtiter well and samples contained the drug will compete for streptomycin specific antibody with the antigen. After washing and adding the HRP conjugate, TMB substrate is used to show the reaction color. The concentration of streptomycin in sample is inversely proportional to the OD value, which can be interpreted with a standard curve.

Key facts of the Streptomycin ELISA Kit

• Ready to use ELISA kit based on Competitive Immunoassay
• Total time required only 60min
• Highly specific to Streptomycin.
• Applicable for milk, milk powder, honey and cell culture sample

Streptomycin ELISA kit parameters

• Sensitivity: 0.2 ppb, ng/ml
• Stability: 12 months when stored properly
• Accuracy: 70%-120%

Streptomycin ELISA kit components

• Precoated microwell, 96wells
• Streptomycin standard series, 0.2 - 51.2 ng/ml
• Enzyme conjugate
• TMB Substrate
• Stop solution (not available when shipping by air)
• Sample buffer
• Wash buffer

Further readings on Streptomycin and Streptomycin testing:

• Granja, R. H., Niño, A. M. M., Zucchetti, R. A., Niño, R. E. M., Patel, R., & Salerno, A. G. (2009). Determination of streptomycin residues in honey by liquid chromatography–tandem mass spectrometry. Analytica chimica acta, 637(1-2), 64-67.
• Baxter, G. A., Ferguson, J. P., O'Conno, M. C., & Elliott, C. T. (2001). Detection of streptomycin residues in whole milk using an optical immunobiosensor. Journal of agricultural and food chemistry, 49(7), 3204-3207.